膜科学与技术

纳滤选择性分离丁二酸模拟发酵液

作者：吴昊，姜岷，韦萍，高超，杨高，雷丹

单位：南京工业大学制药与生命科学学院，材料化学工程国家重点实验室

关键词：丁二酸；纳滤；选择性分离；模拟发酵液46

出版年,卷(期):页码： 2011,31(5):46-51

摘要：

生物法制备的丁二酸发酵液中含有残留的底物(葡萄糖)、甲酸、乙酸及二价无机离子(Mg2+、SO42-)等杂质，不利于分离纯化。采用截留分子量为160Da的纳滤膜LNG-NF-016对丁二酸模拟发酵液进行分离，考察了pH、操作压力、MgSO4浓度、丁二酸浓度、葡萄糖浓度、料液体积流量、操作温度等因素对纳滤分离性能的影响。纳滤分离的选择性由丁二酸的解离状态决定：模拟发酵液调节至pH3.0，丁二酸未解离，可将其与葡萄糖、MgSO4分离，丁二酸的透过率达98%，葡萄糖、MgSO4的截留率均＞85%。随后将透过液调节至pH6.0，丁二酸形成二价阴离子，可将其与单价的甲酸盐、乙酸盐分离，丁二酸截留率达90%，丁二酸被浓缩至121g/L，而膜截留液中的甲酸、乙酸浓度均＜5g/L。同一型号纳滤膜可实现模拟发酵液中丁二酸与副产一元有机酸、底物及多价无机离子的选择性分离。

Many impurities existed in succinic acid fermentation broth, such as by-product organic acids(formic acid, acetic acid), residual substrate(glucose) and divalent ion (Mg2+ , SO42-) , which was unfavorable to purification. Separation of succinic acid from simulated fermentation broth by nanofiltration membrane LNG-NF-016(MWCO 160Da) was studied, For this purpose, the effect of operating parameters such as pH in the feed solution, operation pressure, MgSO4 concentration, succinic acid concentration, glucose concentration, cross-flow rate and operation temperature on nanofiltration was investigated using an experimental design technique. The Selectivity of nanofiltration depended on the degree of dissociation of succinic acid, At pH about 3.0, undissociated succinic acid could be separated from glucose and MgSO4, succinic acid permeation of 98%, glucose and MgSO4 rejection more than 85%,.The Divalent succinate could be separated from acetate and formate at pH about 6.0, succinic acid rejection of 90%, succinic acid concentration about 121 g/L in retentate, formic acid and acetic acid concentrations below 5 g/L. Succinic acid was separated selectively from by-product organic acids, residual substrate and divalent ion in simulated fermentation broth by the same nanofiltration membrane.

吴昊（1976-），男，讲师，南京工业大学，博士研究生，研究方向：发酵工程、生物分离工程*通讯作者：姜岷（1972-），男，教授，南京工业大学，博士，研究方向：发酵工程、酶工程

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