BPPO中空纤维荷电镶嵌膜制备及其蛋白质吸附性能
作者:吴翠明 , 孙夫建1 王娜2,徐铜文2
单位: 1合肥工业大学化工学院 安徽,合肥230009;
关键词: BPPO;巯丙基三乙氧基硅烷;季铵化;荷电镶嵌膜;BSA
分类号: O63,TB33
出版年,卷(期):页码: 2014,34(1):67-73

摘要:
以季铵化BPPO中空纤维膜为原料,通过sol-gel反应填充巯丙基三乙氧基硅烷(KH580),然后进行氧化,得到同时含有季铵和磺酸基团的杂化荷电镶嵌膜,阴离子交换容量(AEC)和阳离子交换容量(CEC)分别为1.57-1.76mmol/g和1.07-1.24mmol/g。对该膜进行了结构和物化性能的表征,然后进行蛋白质吸附和脱附。结果表明,与基膜相比,荷电镶嵌膜在溶胀度方面有所改进。pH= 4,7和8时荷电镶嵌膜对牛血清蛋白(BSA)的吸附量较高,可达到20mg/g以上。在碱性条件下(pH=8),脱附率明显高于基膜,可以达到约95%。由此说明基于BPPO的荷电镶嵌中空纤维膜可以用作吸附材料。
Quaternized BPPO hollow fiber membrane was used as the starting material and modified by sol-gel and oxidization reactions of 3-mercaptopropyltriethoxysilane (KH580), so that ion exchange mosaic hybrid membranes containing -N+(C2H5)3 and -SO3H groups were obtained. The anion and cation exchange capacities (AEC and CEC) were 1.57-1.76mmol/g and 1.07-1.24mmol/g respectively. The structure and physico-chemical properties of the membranes were characterized and then the membranes were applied for protein adsorption and desorption performances. The results showed that the swelling resistance of the ion exchange mosaic membranes was improved as compared with the base membrane. The adsorption capacities for bovine serum albumin (BSA) at pH= 4, 7 and 8 were relatively higher and could reach more than 20mg/g. The desorption capacity under alkaline conditions (pH=8) was obviously higher than the BPPO base membranes and could reach around 95%. The results indicate that the BPPO-based ion exchange mosaic membranes can be potentially used as adsorption materials.

基金项目:
国家自然科学基金项目(No. 21176053, 51003100);安徽省自然科学基金(No. 11040606M37)

作者简介:
吴翠明(1978-),女,副教授,电话.: +86-551-290-1458. E-mail: cmwu@ustc.edu.cn

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