膜科学与技术

海藻酸盐基微囊膜的物质传递性能

作者：王云红，刘袖洞，于炜婷，戴小敏，马小军

单位：（1.大连大学环境与化学工程学院，大连116622； 2.中国科学院大连化学物理研究所，大连116023）

关键词：海藻酸钠；微囊膜；物质传递

出版年,卷(期):页码： 2017,37(2):6-11

摘要：

天然多糖海藻酸钠具有良好的生物相容性、生物降解性、黏附性以及独特的应力形变-释放特性，因此，海藻酸钠外覆聚电解质络合膜形成的微胶囊是很有前途的细胞培养和药物缓控释载体。膜的渗透分离特性决定了海藻酸盐基微囊的物质扩散传递行为，对其在生物医学领域的应用至关重要。本文主要综述了海藻酸盐基微囊膜的传质数学模型研究，概述了小分子物质、大分子蛋白以及氧气在海藻酸盐基微囊膜上的扩散传递行为，总结了材料物化性能、pH值等参数对微囊膜渗透性能的影响规律，对海藻酸盐基微胶囊的制备和应用具有较大的参考价值。

Natural polysaccharide alginate shows good biocompatibility, biodegradability, adhesi-
on, as well as unique deformation stress-release characteristics, so that alginate-based microcapsules with outer polyelectrolyte complex membrane are promising carriers for cell culture and sustained/controlled release. Membrane permeability can decide the diffusion and mass transfer properties of alginate-based microcapsules, which is critical for its application in the biomedical field. We mainly discussed the mathematical models of mass transfer on microcapsule membrane, the diffusion behavior of small molecules, macromolecules (such as proteins) and oxygen in alginate-based microcapsules membrane, summarized the influence rules of parameters such as materials properties, pH on membrane permeability. It is hopeful to provide reference for the preparation and application of alginate-based microcapsules.

第一作者简介：王云红（1990-），女，山西人，硕士生，主要从事高分子材料的研究。* 通讯作者，E-mail:

参考文献：

[1] 刘袖洞, 于炜婷, 王为, 等. 海藻酸钠和壳聚糖聚电解质微胶囊及其生物医学应用[J]. 化学进展, 2008,
20(1): 126−139.
[2] Xu X, Wu X T, Wang Q Q, et al. Immunomodulatory Effects of Alginate Oligosaccharides on Murine Macrop-
hage RAW264.7 Cells and Their Structure−Activity Relationships[J]. Agricultural And Food Chemistry, 2014,
62(14): 3168−3176.
[3] Abraham S M, Vieth R F, Burgess D J. Novel technology for the preparation of sterile alginate-poly-l-lysine
microcapsules in a bioreactor[J]. Pharm Dev Technol, 1996, 1(1): 63−68.
[4] Wang S B, Xu F H, He H S, et al. Novel alginate-poly(L-histidine) microcapsules as drug carriers: In vitro
protein release and short term stability[J]. Macromolecular Bioscience, 2005, 5(5): 408−414.
[5] Halder A, Mukherjee S, Sa B. Development and evaluation of polyethyleneimine-treated calcium alginate
beads for sustained release of diltiazem[J]. Journal of Microencapsulation, 2005, 22(1): 67−80.
[6] Hearn E, Neufeld R J. Poly(methylene co-guanidine) coated alginate as an encapsulation matrix for urease
[J]. Process Biochemistry, 2000, 35(10): 1253−1260.
[7] Markvicheva E A, Kuptsova S V, Mareeva T Y, et al. Immobilized enzymes and cells in poly(N-vinyl caprolac-
tam)-based hydrogels-Preparation, properties, and applications in biotechnology and medicine[J]. Applied
Biochemistry and Biotechnology, 2000, 88(1−3): 145−157.
[8] Huguet M L, Neufeld R J, Dellacherie E. Calcium alginate beads coated with polycationic polymers:Comparis-
on of chitosan and DEAE-dextran[J]. Process Biochemistry, 1996, 31(4): 347−353.
[9] Chen J P, Chu I M, Shiao M Y, et al. Microencapsulation of islets in PEG-amine modified alginate-poly(L-
lysine)-alginate microcapsules for constructing bioartificial pancreas[J]. Journal of Fermentation and Bioengi-
neering, 1998, 86(2): 185−190.
[10] 薛伟明, 于炜婷, 刘袖洞, 等. 载细胞海藻酸钠/壳聚糖微胶囊的化学破囊方法研究[J]. 高等学校化学
学报, 2004, 25(7): 1342−1346.
[11] 何荣军, 杨爽, 孙培龙, 等. 海藻酸钠/壳聚糖微胶囊的制备及其应用研究进展[J]. 食品与机械, 2010,
2(26):166−169.
[12] 蔡涛, 王丹, 宋志祥, 等. 微胶囊的制备技术及其国内应用进展[J]. 化学推进剂与高分子材料, 2010,
8(2): 20−26.
[13] Qi W T, Ma J, Ma X J, et al. Insight into permeability of protein through microcapsule membranes[J]. Journal
of Membrance Science, 2006, 269: 126−132.
[14] Song Kedong, Li Liying, Li Ruipeng, etal. Preparation, Mass Diffusion, and Biocompatibility Analysis of
Porous–Channel Controlled Calcium–Alginate–Gelatin Hybrid Microbeads for In Vitro Culture of NSCs[J].
Biochem Biotechnol, 2014,173:838−850.
[15] Yao S J, Cho M G. Diffusion Characteristics in Microcapsules[J]. Chinese Journal of Chemical Engineering,
1998, 6 (2): 116−123.
[16] 朱敏莉, 薛伟明. 海藻酸钠−壳聚糖−海藻酸钠液芯微胶囊的传质及生物微囊化[J]. 食品与发酵工业,
2010, 36(4): 11−14.
[17] 张宏亮, 薛伟明, 赵彬然, 等. 壳聚糖/海藻酸盐微胶囊膜的物质扩散性能研究[J]. 膜科学与技术,2009,
29(2): 77−80.
[18] 孙学战, 刘源岗, 王士斌, 等. 高载药量海藻酸钙/几丁聚糖微胶囊的性能[J]. 化工进展, 2010, 29(9):
1725−1729.
[19] 刘映薇, 于炜婷, 刘袖洞, 等. 海藻酸钠−壳聚糖−海藻酸钠(ACA)微胶囊的蛋白质通透性研究[J]. 中国
生物医学工程学报, 2006, 25(3): 371−373.
[20] 谢红国, 李晓霞, 于炜婷, 等. 壳聚糖脱乙酰度对海藻酸钠/壳聚糖微胶囊的表面性质及蛋白吸附的影
响[J]. 复合材料学报, 2011, 28(2): 111−116.
[21] 赵伟, 张英, 于炜婷, 等. 海藻酸钠−壳聚糖复合膜中溶氧扩散性能研究[J]. 高等化学工程学报,2012,
26(3): 429−435.
[22] Sharp N A, Daugulis A J, Goosen M F A, etal. Hydrodynamic and mass transfer studies in an external−loop
air−lift bioreactor for immobilized animal cell culture[J]. Applied Biochemistry and Biotehnology, 1998,73:
59−77.
[23] 刘博, 张宏亮, 薛伟明, 等. 载脂肪酶壳聚糖/海藻酸钙微胶囊的制备[J]. 化工进展, 2010, 29(8):1541−
1546.
[24] 姜恒丽，崔元璐，齐学洁，等. 海藻酸钠−壳聚糖微胶囊载体在组织工程研究中的应用[J]. 中国组织工
程研究, 2014, 18(3) :412−419.
[25] 徐旻, 李琳, 于长青. 植物乳杆菌M1−UVs29缓释微胶囊的制备及其缓释性能分析[J]. 2015, 28(7):
723−728.
[26] Yu Weiting, Song Huiyi, Zheng Guoshuang, etal. Study on membrane characteristics of alginate−chitosan
microcapsule With cell growth[J]. Journal of Membrane Science, 2011, 377: 214−220.
[27] 吴文果, 刘伟, 王士斌, 等.海藻酸钙/聚精氨酸微胶囊的载药和缓释性能[J]. 化工进展，2014, 35(5):
1271−1275.
[28] 王慧萍, 丁妮, 周佑亮, 等. 基于气−液剪切作用制备壳聚糖季铵盐/海藻酸钠/CaCO3复合微胶囊及其
性能研究[J]. 高分子学报, 2015, (11): 1336−1343.
[29] 冯志云, 吴敏, 朱昌平, 等.聚乙二醇共价交联海藻酸钠凝胶制备及其药物缓释性能[J]. 材料科学与工
程学报, 2015, 33(3): 368−424.
[30] 唐文, 周凤, 姜彪, 等. 猪脾脏转移因子壳聚糖−海藻酸钠微囊的制备及其性能[J]. 过程工程学报,
2011, 11(4): 660−665.
[31] 张守庆, 王宝杰, 姜珊, 等.重组抗菌肽海藻酸钠微囊制备与体外释放特征研究[J]. 海洋科学, 2012, 36
(4):1−6.
[32] He Fan, Mei Li, Ju Xiaojie, etal. pH−responsive controlled release characteristics of solutes with different
molecular weights diffusing across membranes of Ca−alginate/protamine/silica hybrid capsules[J]. Journal of
Membrane Science, 2015, 474: 233−243.
[33] Mei L, He F, Zhou R Q, etal. A novel intestinal−targeted Ca−alginate−based carrier for pH−responsive protec-
tion and release of lactic acid bacteria[J]. ACS Appl.Mater. Interfaces, 2014, 6(8): 5962−5970.
[34] Wu Jun, Kong Tiantian, Wang Liqiu, etal. Fabrication and characterization of monodisperse PLGA−alginate
core−shell microspheres with monodisperse size and homogeneous shells for controlled drug release[J]. Acta
Biomaterialia, 2013, 9: 7410−7419.
[35] 李思阳, 郑健, 骆沙曼, 等.10−羟基喜树碱的聚赖氨酸/海藻酸钠微胶囊的制备及其体外释药特性研
究[J]. 中草药, 2011, 42(9): 1724−1727.
[36] 向飞, 颜慧琼, 陈秀琼, 等. 海藻酸钠/壳聚糖层层裹覆凝胶微球的缓释性能[J]. 化工新型材料, 2016,
44(2): 165−168.
[37] 李思阳. 红景天苷缓释微胶囊的制备及体外释放特性研究[J]. 中药与临床, 2014, 5(6): 19−20.
[38] 宇佳, 关桦楠, 田可, 等. 杀铃脲缓释微胶囊制备表征及缓释性能的研究[J]. 食品工业科技. 2013, 34
(19): 104−107.
[39] 田可, 迟德富, 张喆. 灭幼脲缓释微胶囊的制备与性能[J]. 农药, 2012, 50(3): 184−186.

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