左氧氟沙星共混PVDF抑菌超滤膜的制备及性能表征
作者:廖祥军,刘海龙
单位: (山西大学环境与资源学院,太原 030006)
关键词: 左氧氟沙星;PVDF;亲水性;抗污染;抑菌
分类号: TQ028.8
出版年,卷(期):页码: 2018,38(5):38-46

摘要:
 采用浸没沉淀相转化法制备了左氧氟沙星(Levofloxacin,LVFX)共混聚偏氟乙烯(PVDF)超滤膜,考察了左氧氟沙星作添加剂对膜结构及性能的影响。采用傅里叶变换红外光谱(FTIR)、扫描电镜(SEM)和接触角等表征手段,分析了左氧氟沙星在PVDF膜上存在及其对膜结构和亲水性能的影响规律。分别利用牛血清蛋白(BSA)静态吸附、循环通量衰减和抑菌圈、抑菌率评价了LVFX/PVDF共混膜的抗污染性能和抑菌性能。结果表明,左氧氟沙星稳定存在于膜上;随着左氧氟沙星添加比的增加,共混膜的接触角由77.7°下降至62.1°,亲水性增强;共混膜的BSA静态吸附量下降,通量恢复率升高,不可逆污染指数在LVFX添加比为0.6% 时,降至11.8%,抗污染能力最佳;LVFX添加比为0.3%时,即有抑菌圈生成,添加比为0.6% 时,抑菌率可达92.3%,抑菌性能优异。此外,一段时间运行的共混膜经再生后,仍能保持较好的抑菌效果。
 Levofloxacin(LVFX)/polyvinylidene difluoride(PVDF) blend ultrafiltration membrane was prepared by immersion-precipitation phase inversion method. The effects of levofloxacin as additive on membrane structure and properties were investigated. Fourier transform infrared spectroscopy (FTIR)、scanning electron microscope (SEM) and contact angle were used to characterize the existence of levofloxacin on PVDF membrane and its influence on membrane morphology and hydrophilicity. The antifouling performance and antimicrobial activity of LVFX/PVDF blend membrane were evaluated respectively by static adsorption of BSA, decay of circulating flux, inhibition zone and inhibition rate. The results showed that levofloxacin was present on the membrane stably. With the increase of the levofloxacin ratio, the contact angle of the blend membrane decreased from 77.7°to 62.1°, the hydrophilicity of the membrane was enhanced. The BSA static adsorption capacity decreased while the flux recovery rate increased, the irreversible pollution index decreased to 11.8% when the addition ratio of LVFX was 0.6%, which possessed the best antifouling ability. The inhibition zone was formed when the addition ratio of LVFX was 0.3%. When the adding ratio was 0.6%, the antibacterial rate could reach 92.3%, showing excellent antibacterial properties. In addition, the blend membranes was regenerated after run for a period of time, still maintained a good antibacterial effect.

基金项目:
家自然科学基金项目(51179099/E090301);山西省重点研发计划项目(201603D321007);山西省科技攻关项目(20140313003-3);山西省留学回国人员科技活动择优资助项目(2014);山西省回国留学人员科研资助项目(2015-004)

作者简介:
第一作者简介:廖祥军(1995-),男,山东泰安人,硕士研究生,从事膜材料制备及其应用研究,E-mail:xiangjunliao@yeah.net. *通讯作者,E-mail:hlliu827@aliyun.com

参考文献:
 [1] 时钧, 袁权, 高从堦. 膜技术手册[J]. 2001, 28(19):127-127.
[2] 王剑, 高学理, 王汉敏,等. 抑菌性聚醚砜超滤膜的制备及性能表征[J]. 膜科学与技术, 2013,33(3):12-16.
[3] Chang H H, Chen S C, Lin D J, et al. The effect of Tween-20 additive on the morphology and performance of PVDF membranes[J]. J Membr Sci, 2014, 466(466):302-312.508.
[4] Silva T L S, Morales-Torres S, Figueiredo J L, et al. Multi-walled carbon nanotube/PVDF blended membranes with sponge- and finger-like pores for direct contact membrane distillation[J]. Desalination, 2015,357:233-245.
[5] 赵爱桔. 左氧氟沙星合成工艺的研究[D]. 浙江大学, 2011.
[6] 王艳波. 甲磺酸左氧氟沙星不对称合成工艺的研究[D]. 天津大学, 2006.
[7] 陈金月, 文隽, 龚敏阳,等. 不同光照对盐酸左氧氟沙星注射液稳定性的影响[J]. 中国药 房,2005,16(6):459-460.
[8] 朱小梅. 氧氟沙星的热稳定性及其热分解动力学[J]. 化学世界, 2008, 49(6):333-335.
[9] Blokhina S, Sharapova A, Ol’Khovich M, et al. Sublimation thermodynamics of four fluoroquinolone antimicrobial compounds[J]. J Chem Thermodyn, 2017, 105:37-43.1
[10] 王艳萍, 史沛海, 孟庆彪,等. 盐酸左氧氟沙星注射液的稳定性实验[J]. 解放军药学学报, 2002,18(6):381-382.
[11] 顾洪安, 沈浩, 张曙华,等. 左氧氟沙星注射剂的稳定性[J]. 中国临床药学杂志, 2007,16(5):311-314.1
[12] 杨学智. 左氧氟沙星溶解度测定及共晶点的分析研究[D]. 天津大学, 2012.
[13] 彭湘红, 陈春华. 盐酸左氧氟沙星壳聚糖药物膜的制备及性能研究[J]. 湖北大学学报(自科 版),2004,26(2):147-150.
[14] Khan G, Yadav S K, Patel R R, et al. Development and Evaluation of Biodegradable Chitosan Films of Metronidazole and Levofloxacin for the Management of Periodontitis[J]. Aaps Pharmscitech, 2016, 17(6):1-14.
[15] 彭湘红, 王敏娟, 陆茜,等. 氧氟沙星-壳聚糖-明胶共混膜的制备及表征[J]. 精细化 工,2000,17(6):325-327.
[16] 赵铁, 杜予民, 唐汝培. 壳聚糖水杨酸盐-明胶共混膜结构表征及其抗菌性[J]. 分析科学学报, 2002, 18(2):100-104.
[17] 甄洪鹏, 杨冬芝, 郭爽,等. 壳聚糖/聚乙烯醇/氧氟沙星共混无纺布的制备及体外释放研究[J]. 北京化 工大学学报(自然科学版), 2007, 34(3):271-274.
[18] Islan G A, Tornello P C, Abraham G A, et al. Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery. Design and characterization[J]. Colloid Surface B , 2016, 143:168-176.
[19] 王婷, 沈明谦, 李平,等. 氧氟沙星壳聚糖胶囊的稳定性研究[J]. 兰州大学学报(医学版), 2002,28(3):41-43.
[20] Jalvandi J, White M, Gao Y, et al. Slow release of levofloxacin conjugated on silica nanoparticles from poly(É?-caprolactone) nanofibers[J]. Int J Polym Mater,2017,66(10):507-513. .
[21] 于慧, 张梦, 宋杰,等. 膜抗菌性评价方法概述[J]. 膜科学与技术, 2015, 35(4):123-129.
[22] Huang L, Zhao S, Wang Z, et al. In situ immobilization of silver nanoparticles for improving permeability,antifouling and anti-bacterial properties of ultrafiltration membrane[J]. J Membr Sci,2016,499:269-281.
[23] Yu L, Zhang Y, Zhang B, et al. Preparation and characterization of HPEI-GO/PES ultrafiltration membrane with antifouling and antibacterial properties[J]. J Membr Sci, 2013, 447(22):452-462.
[24] 陈浩, 李瑞林, 许顺贵. 左氧氟沙星片的溶出度考察[J]. 解放军药学学报, 2001, 17(5):288-289.
[25] Wang H, Wang Z M, Yan X, et al. Novel organic-inorganic hybrid polyvinylidene fluoride ultrafiltration membranes with antifouling and antibacterial properties by embedding N-halamine functionalized silica nanospheres[J]. J Ind Eng Chem, 2017, 52:295-304.
[26] Lü X, Wang X, Guo L, et al. Preparation of PU modified PVDF antifouling membrane and its hydrophilic performance[J]. J Membr Sci, 2016, 520:933-940.
[27] Yuan H, Ren J. Preparation of Poly(vinylidene fluoride) (PVDF)/Acetalyzed Poly(vinyl alcohol) Ultrafiltration Membrane with the Enhanced Hydrophilicity and the Anti-fouling Property[J]. Chem Eng Res Des, 2017, 121.
[28] Chen X, Su Y, Shen F, et al. Antifouling ultrafiltration membranes made from PAN-b-PEG copolymers:Effect of copolymer composition and PEG chain length[J]. J Membr Sci, 2011, 384(1–2):44-51.
[29] 徐又一, 徐志康. 高分子膜材料[M]. 材料科学与工程出版中心, 2005.

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