膜科学与技术

万古霉素手性膜色谱的研究

作者：艾萍，杜洋，杨娜，袁黎明

单位：云南师范大学化学化工学院，昆明市 650500

关键词： D,L-苯甘氨酸；万古霉素；手性膜；手性膜色谱；手性分离

出版年,卷(期):页码： 2020,40(2):1-5

摘要：

以聚醚砜膜作为基膜，以万古霉素、1,6-己二异腈酸酯作为单体，利用界面聚合的方法制备了万古霉素-己二异腈酸酯聚醚砜手性高分子复合膜，使用自制的膜色谱装置结合高效液相色谱仪，对手性物质D,L-苯甘氨酸进行了手性膜色谱分离研究。该文详细研究了流速、流动相、进样量、膜的层数、膜的尺寸对拆分效果的影响。在优选分离条件下，该手性膜色谱法对D,L-苯甘氨酸拆分的分离因子(α)和分离度(Rs )分别为5.66、0.66，该方法的最大优点是在水环境下能对手性化合物进行分离制备，这在色谱的手性分离中是少见的。

Using vancomycin and 1,6-diisocyanatehexane as monomers, an enantioselective composite membrane was prepared by interfacial polymerization on a polyethersulfone support. After the composite membrane was mounted in a home-made device of membrane chromatography for high performance liquid chromatography, the enantioseparation of D,L-phenylglycine was carried outed by the chiral membrane chromatography. By optimizing the injection volume, the flow rate, the constituent of mobile phase, the size of the membrane, the layer numbers of the membrane, over 5.66 of separation factor (α) and 0.66 of resolution (Rs ) could be obtained, respectively. The advantage of the method is the ability to separate and prepare chiral compounds in an aqueous environment, which is rare in chromatographic chiral separation.

第一作者简介：艾萍（1962-），女，四川高县人，教授，从事手性分离研究，E-mail：aipdeyx@aliyun.com 通讯作者：E-mail：yuan_limingpd@126.com

参考文献：

[1] Wang L, Ghosh R. Feasibility study for the fractionation of the major human immunoglobulin G subclasses using hydrophobic interaction membrane chromatography [J]. Anal Chem, 2010, 82: 452-455.[2] Madadkar P, Umatheva U, Hale G,et al. Ultrafast separation and analysis of monoclonal antibody aggregates using membrane chromatography [J]. Anal Chem, 2017, 89: 4716-4720.
[3] 刘丛丛,裴洪昌,刘耀龙,等. 氨基苯并-15-冠-5接枝聚砜多孔膜填充膜色谱构建及锂同位素吸附分离性能研究[J].膜科学与技术,2018,38(4),14-22.
[4] Osada Y, Ohta F, Mizumoto A, et al. Specific permeation and adsorption of aqueous amino acids by plasma-polymerized membranes of d-camphor and l-menthol [J]. Journal of the Chemical Society of Japan, 1986, (7): 866-872.
[5] Yang M, Chu L, Wang H, et al. A thermoresponsive membrane for chiral resolution [J]. Adv Funct Mater, 2008, 18(4): 652-663.
[6] Xie R, Chu L Y, Deng J G. Membranes and membrane processes for chiral resolution [J]. Chem Soc Rev, 2008, 37(6): 1243-1263.
[7] Wang W J, Dong X L, Nan J P, et al. A homochiral metal-organic framework membrane for enantioselective separation [J]. Chem Commun, 2012, 48(56): 7022-7024.
[8] Kang Z X, Xue M, Fan L L, et al. “Single nickel source” in situ fabrication of a stable homochiral MOF membrane with chiral resolution properties [J]. Chem Commun, 2013, 49(90): 10569-10571.
[9] Meng C C, Chen Q B, Liu H L, et al. Role of filled PLGA in improving enantioselectivity of Glu-GO/PLGA composite membranes [J]. J. Membr Sci, 2018, 555: 398-406.
[10] Higuchi A, Tamai M, Ko Y A, et al. Polymeric membranes for chiral separation of pharmaceuticals and chemicals [J]. Polym Rev, 2010, 50(2):113-143.
[11] Yoshikawa M, Tharpa K, Dima S O. Molecularly imprinted membranes: past, present, and future [J]. Chem Rev, 2016, 116(19): 11500-11528.
[12] 艾萍,张紫恒,袁黎明. 金属－有机骨架材料[Zn2Camph2(dabco)].DMF.H2O作为手性选择剂的高分子膜及其应用研究[J].膜科学与技术, 2018, 38(5): 84-87.
[13] Yuan L M, Ma W, Xu M, et al. Optical resolution and mechanism using enantioselective membranes of cellulose, sodium alginate and hydroxypropyl-β-cyclodextrin [J]. Chirality, 2017, 29:

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