膜科学与技术

电催化膜灭活大肠杆菌机制研究

作者：王尹，赵蕾，邓橙，李双戎，程晓，刘红斌，朱孟府

单位：军事科学院系统工程研究院卫勤保障技术研究所，天津 300161

关键词：电催化膜；大肠杆菌；灭活；氧化；损伤机制

出版年,卷(期):页码： 2022,42(2):34-39

摘要：

通过电催化膜对大肠杆菌进行灭活处理，采用电子顺磁共振技术检测电催化过程产生的自由基，采用平板计数、电镜观察、双染法和刃天青还原实验等方法评价大肠杆菌的可培养能力、形态结构、细胞膜通透性和新陈代谢能力，探究电催化膜灭活大肠杆菌的机制。研究结果表明，电催化膜在水处理过程中能够产生对灭活大肠杆菌起重要作用的·OH、1O2等氧化活性物质，能破坏大肠杆菌的结构形态、细菌还原酶和新陈代谢能力，使细菌失去活性而灭活；大肠杆菌灭活效果随着电催化膜电流密度的增加而增加，当电流密度增加到75 mA·cm-2时，大肠杆菌减少了4.1个数量级，具有新陈代谢能力的活细菌百分比下降到了32.4%。

In this study, an electrocatalytic membrane was used to inactivate E. coli and the mechanism was identified through a series of technical methods. The EPR measurement was used to detect free radicals produced during the electrocatalysis process. The plate counting, electron microscope observation，dual staining，resazurin reduction test and other technical methods were used to determine the cultivability, morphological structure, cell membrane permeability and metabolic capacity of Escherichia coli. These results showed that the electrocatalytic membrane can produce reactive oxygen species such as hydroxyl radicals and singlet oxygen during the treatment process, and can destroy the structure, bacterial reductase and metabolism of E.coli, thus endowing it the ability of inactivating bacteria. The effect of the electrocatalytic membrane for inactivating E. coli increased with the increase of current density. When the current density reached 75 mA·cm-2, the total number of E. coli colonies decreased by 4.1 orders of magnitude, and the percentage of living bacteria with metabolic ability decreased to 32.4%.

王尹（1997-），男，江苏苏州人，硕士生，研究方向为膜分离净化技术，E-mail：1224582874@qq.com

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