膜科学与技术

利用纳滤膜技术分离秸秆发酵液中糖和乳酸的研究

作者：郭佩，赵黎明，刘鲁杰，邱勇隽，李爽

单位： 1 华东理工大学生物工程学院发酵工业分离提取技术研发中心生物反应器工程国家重点实验室，上海 200237;2 上海冠生园食品有限公司，上海 200237

关键词： L-乳酸；残糖；秸秆发酵液；纳滤；CFSK模型

出版年,卷(期):页码： 2023,43(3):104-115

摘要：

本文旨在研究以秸秆类生物质为原料的L-乳酸发酵液中L-乳酸与残糖的纳滤分离工艺和特性。基于特有的高浓度L-乳酸发酵液的多组分体系，考察了pH值、跨膜压差、温度和离子强度对L-乳酸与残糖纳滤分离的影响，并且成功开发了糖/酸三级纳滤分离工艺。在最优纳滤条件（pH=2.00、压力2 MPa、温度25 ºC）下，乳酸纯度(质量分数)由92.4%提升至99.7%，每级纳滤的乳酸收率均高于80.0%，同时还达到了理想的脱色效果。此外，在机理方面，将CFSK模型（Combined Film-theory SKK model）的内、外插值结果与实验数据进行比对,证实了CFSK模型用于描述秸秆发酵液体系中糖/酸纳滤分离的适用性和准确性。

This study aims to investigate the nanofiltration membrane separation technology to separate the residual sugars and the L-lactic acid from straw biomass fermentation broth. Based on the unique multi-component fermentation broth system with high concentration of L-lactic acid, the effects of pH, transmembrane pressure, temperature and ionic strength on the separation of residual sugars and L-lactic acid were investigated. Based on the optimal nanofiltration conditions (pH=2.00, 2 MPa, 25 ºC), a three-stage nanofiltration separation process was designed to perform nanofiltration separation on the actual fermentation broth. The purity (wt/wt %) of L-lactic acid was increased from 92.4% to 99.7%. The yield of L-lactic acid was higher than 80.0% in each stage, and the ideal effect of decolorization were achieved simultaneously. In terms of mechanism, the applicability and accuracy of CFSK model in describing nanofiltration separation of lactic acid and residual sugar in the straw fermentation broth system were established for the first time by comparing the internal and external interpolation results of the model with the experimental data.

郭佩（1994-），女，湖北黄冈人，硕士，从事纳滤膜分离研究，E-mail：18616011302@163.com。

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