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pH–resopnsive and protein separation properties of intelligent switch ethylene-co-vinyl alcohol copolymer membrane

Authors： YANG Bin1,2, YE Hui1,2, LIU Qi1,2, LI An-ni1,2, ZHANG Yu-zhong1,2, ZHAO Li-zhi1,2, LI Hong1,2

Units： 1. School of Material Science and Engineering，Tianjin Polytechnic University Tianjin 300387，China；2. State Key Laboratory of Hollow Fiber Membrane Materials and Processes，Tianjin Polytechnic University，Tianjin 300387，China

KeyWords： intelligent switch membrane; pH-responsive;ethylene-vinyl alcohol copolymer; protein separation

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2016,36(3):48-53

Abstract：

Membrane technique used for protein separation has gained great attention in the life science field.Intelligent switch membranes provide an efficient method for efficient protein separation. In this research, intelligent switch ethylene vinyl alcohol copolymer (EVAL) membranes were prepared by grafting dimethylaminoethylmethacrylate(DMAEMA) using UV irradiation graft polymerization.ThepH–responsive and protein separation properties of the intelligent switch membranes were investigated. The results indicated that the pH-responsive “on/off” properties of the grafted EVAL membrane are observed. During six cyclic measurements, the pH-responsive “on/off” properties are also observed. During the separation of bovine serum albumin (BSA) / lysozyme (LZM) model solution andBSA/LZM binary mixture, the highest separation factoris achieved at pH 3.6 of poly(DMAEMA)-grafted EVAL membrane with GD 15%.The separation factor values are 9.7 and 7.8, respectively.

Funds：

国家自然科学基金资助项目（51373120,51173132,21204064）；高等学校博士学科点专项科研基金（20111201110003）；教育部团队；中空纤维膜材料与膜过程省部共建国家重点实验室培育基地开放课题；天津市大学生创新创业训练计划项目（201410058082）

AuthorIntro：

第一作者：杨彬（1989—），男，硕士研究生，天津人，主要研究方向为功能膜分离。通信作者： E-mail：qianye325326@163.com

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